Heyrovsky Institute of Physical Chemistry of the CAS, v. v. i.
Eliska Mikyskova
+420 26605 3039
eliska.mikyskova@jh-inst.cas.cz
Dolejškova , 2155/3, Praha 8, 182 23
High-performance liquid chromatograph (HPLC) with an autosampler (UFCH35) (Pro-NanoEnviCzIII)
Liquid chromatography is one of the key separation and analytical techniques. It plays a crucial role in addressing the degradation of new types of water contaminants and in developing new nanotechnologies for environmental protection. The instrument is equipped with an HPLC quaternary pump with a pressure of up to 1050 bar, pH tolerance from 1 to 14, and a flow rate of 0.0001–10 mL/min. It features an autosampler with pressure resistance up to 1050 bar. The injection volume ranges from 0.1 to 50 µL, with injection linearity of ≥ 0.9999. The autosampler has a capacity of 162 standard vials (12 mm, volume 2 mL). Samples in the autosampler can be thermostated at 4–45 °C. The PDA detector has a wavelength range of 190–800 nm with a maximum sampling frequency of 100 Hz. Wavelength accuracy is ≤ ± 1 nm, and wavelength reproducibility is ≤ ± 0.1 nm. The MS detector is a single quadrupole type, using ESI + APCI ionization (a DUIS source). The m/z range is from 2 to 2000, with a scanning speed of 15000 Da/s and a polarity switching speed of 10 ms. Mass resolution is ± 0.7 Da, with mass determination accuracy of ± 0.1 Da.